FastQCFastQC Report
Mon 27 Feb 2023
SRR3126790.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126790.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901101
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144170.758350029798522No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40810.2146650809188991No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32030.16848131687900852No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21150.11125132226010087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52350.084.995861
ATAGGGC10600.071.622193
GGGCGAT9350.071.368326
TAGGGCA11300.071.358434
CGTAGGG6550.071.2724152
TAGAGGG34950.070.428912
GGTAAGG11800.070.377431
AGTACGG2500.069.833611
AGGGATG26900.069.353275
ATAGAGG14300.068.96251
ATGAGGG31900.068.884712
AGTAGGG35600.068.613152
TATAGGG16200.068.403842
TAGGGCG3600.068.1127554
CGAAGGG17850.067.892612
GTACGGG4950.067.6364442
GAGGGAT22000.067.5173654
GTAGGGC9650.067.4340743
TAAGAGG14850.066.408341
ATAGGGA19750.066.375123
Produced by FastQC (version 0.11.5)