FastQCFastQC Report
Mon 27 Feb 2023
SRR3126789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900343
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135910.71518667945734No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41380.21775016404933217No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32240.16965358358990984No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20340.10703330924996173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48700.083.659341
ATAGCGG4250.076.381061
GGTAAGG10450.072.933021
TAGCGGG11450.071.855622
GTAGGGC9450.070.6195143
AGTAGGG32250.070.55762
TATAGGG16350.069.586632
AACGAGG7750.069.20361
CGTAGGG5800.068.899992
TAGAGGG34050.068.760722
ACGGGAT5700.068.433924
AGGGATG25900.068.4084555
ATGAGGG30350.068.313742
TAGGGCA11250.068.0931244
GAATAGG13450.067.858611
CGAAGGG19650.067.7098852
AGGGCAT19100.067.6655355
GAGGGAT25400.067.534764
ATAGGGC11150.066.5963443
TAGGGTC5600.066.299044
Produced by FastQC (version 0.11.5)