FastQCFastQC Report
Mon 27 Feb 2023
SRR3126788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993814
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142110.7127545498226013No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41410.20769239257021968No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31390.15743695249406414No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20290.10176475839772416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53450.084.944871
CGTAGGG6550.074.6188052
TAGGGCA11850.072.180544
ATAGCGG4250.071.958481
AGGGATG28300.070.744125
AGGGCAT18950.069.4415
TAGCGGG11150.069.123422
CGAAGGG18950.068.943272
GTAGGGC10300.068.896323
ATAGGGC12700.068.4580153
ATGAGGG30150.068.2720642
GAATAGG13100.068.2401351
TAGAGGG35000.067.942062
ACGGGAT6800.067.73054
AAGGGCA30200.067.6938864
AGTAGGG35450.067.212182
TAAGGGA16900.066.183013
ATAGAGG12650.065.832491
GAGGGAT24900.065.6821064
ATAGGGA19250.065.671633
Produced by FastQC (version 0.11.5)