FastQCFastQC Report
Mon 27 Feb 2023
SRR3126787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477251
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217530.8781104538861826No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56490.22803502753657182No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34870.1407608675907286No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC27780.11214043308489935TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG27380.11052574002392168No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27090.10935508755471288No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG26020.10503578361659759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74450.085.298711
ACGGGAT5750.073.793834
CGTAGGG6400.073.665712
TAGCGGG15500.073.608682
TAGGGCA12350.073.29594
AAGGGTA15850.072.875624
AGTAGGG42000.071.729362
GTACGGG5800.071.531952
ATAGGGC14800.070.719093
AGGGCAT21450.070.544055
GGTAAGG14850.070.5323941
ATAGCGG5650.070.144391
CGAAGGG25750.070.124042
AGGGATG37200.069.605015
AGTAAGG14600.068.831761
ACGGGTA1850.068.807774
TAAGGGA20900.068.576093
CGGGAAT5850.068.280215
AATGCGG7000.068.074651
ATAGAGG19300.067.959411
Produced by FastQC (version 0.11.5)