FastQCFastQC Report
Mon 27 Feb 2023
SRR3126786.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126786.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478189
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209550.8455771533163936No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT55490.2239135110356797No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33400.1347758383238728No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC28930.11673847313501916TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG27290.11012073736103259No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG27280.11008038531363024No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25690.10366440977665546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70600.084.194291
ACGGGAT5550.077.9049454
CGTAGGG4900.076.784022
TAGCGGG12000.072.8968352
AGGGATG35000.072.1068345
AGGGCAT22300.072.0761265
AGTAGGG41850.071.472442
ATAGGGC14150.071.4088753
CGAAGGG24700.071.211752
GGTAAGG15400.071.206431
ACGGGTA2450.070.975074
ATAGGGT11900.069.903113
ATAGAGG17550.069.723661
TAGGGTC6000.069.712224
AAGGGTA19700.069.421974
GTAGGGC10800.068.754883
TAGGGCA13500.068.580814
AAGGGAC23350.068.4324654
GAATAGG15050.068.171681
AGGGACT38200.068.157965
Produced by FastQC (version 0.11.5)