FastQCFastQC Report
Mon 27 Feb 2023
SRR3126785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630454
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219010.832593917247745No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57350.21802320055777444No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35820.13617421175203978No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC30450.11575948486458991TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG29320.11146364848045243No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG28310.10762400711055962No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26750.10169347192537867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2050.087.113164
CGTTTTT76900.083.472831
ACGGGAT6450.080.875564
AGGGTAC10900.077.606855
AAGGGTA17400.074.004034
TAGCGGG14400.071.798312
CGTAGGG6950.071.676222
AATGCGG6800.071.2829061
AGTAAGG15650.069.463181
AGGGATG38050.069.4121865
AGGGCAT22350.069.388975
TAGGGCA13850.069.220454
TATAGGG21750.068.7103042
TAGGGTC7000.068.4788064
ATAGGGC16750.068.458773
AGTAGGG44500.068.3279342
TAGACGG3600.067.9763641
CGAAGGG24100.067.860372
ATAGAGG17700.067.533231
GGTAAGG14800.067.411071
Produced by FastQC (version 0.11.5)