FastQCFastQC Report
Mon 27 Feb 2023
SRR3126784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550268
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218840.8581058931845594No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT62540.24522912886018253No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35100.13763259390777752No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27670.1084984009523705No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27620.10830234312629104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81700.084.871061
CGTAGGG7050.081.580672
TAGCGGG12600.072.959292
GGGCGAT12200.072.415756
ACGGGTA2150.072.356074
AGGGATG37050.071.53625
TAAGGGA24150.071.443623
TAGAGGG42850.070.961942
TAGGGCA11550.070.201434
GTAGGGT9900.069.997363
AGTAGGG40750.069.875652
ATAGGGC14900.069.604273
GGTAAGG16100.069.143361
AGAGGGC32300.068.741323
AGGGCAT22600.068.6183855
AATAGGG36250.068.015862
CGAAGGG25600.067.583812
AGGGTAT14750.067.542765
AGTAAGG15300.067.517611
ATAGAGG17800.067.309761
Produced by FastQC (version 0.11.5)