FastQCFastQC Report
Mon 27 Feb 2023
SRR3126783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553184
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210440.8242257510622032No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61640.24142404151052177No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33940.13293205660069937No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27340.10708198077381027No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26450.10359613721533582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81500.083.9311141
CGTAGGG7800.072.939062
ATAGGGC15900.072.415933
AGAGGGC34650.072.156193
TAGGGCG4850.070.7369464
TAGGGCA12350.070.019034
AGGGATG34300.069.878135
TAAGGGA21350.069.339053
AGTAGGG36900.068.9350742
CGAAGGG25600.068.8748552
AGTAAGG15450.068.817071
ATAGAGG18150.068.688721
GGTAAGG14800.068.0249561
AGGGCAT24550.067.958235
GTAGGGT7900.067.817693
TATAGGG22300.067.6814352
AATAGGG36650.067.480942
AGGGCGA14000.067.4734955
ACGGGAT6650.067.13784
TAGCGGG12700.067.010712
Produced by FastQC (version 0.11.5)