FastQCFastQC Report
Mon 27 Feb 2023
SRR3126782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692799
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221530.8226755877434594No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT64750.24045612019315218No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34530.12823088540956826No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27750.10305262293992237No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG27240.10115868284264812No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27060.10049023339655132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80850.084.058091
CGTAGGG7700.079.954592
ACGGGTA2600.077.72454
ATAGCGG4800.072.557591
GACCGAT2500.071.434248
ATAGGGC16350.070.997333
AGGGCAT25600.070.6778955
TAGGGCA13250.069.518924
AGTAGGG42850.069.424982
TAGCGGG14700.067.776852
AGGGATG35300.067.7650765
AGAGGGC34350.067.723793
TAAGGGA23750.067.6745453
GTAGGGC12850.067.659913
AAGAGGG88900.067.34892
GGTAAGG18300.067.124611
ATAAGGG41050.066.97392
AACGAGG11200.066.8146061
GGGCGAT11800.066.511596
AGAAGGG95950.066.416752
Produced by FastQC (version 0.11.5)