FastQCFastQC Report
Mon 27 Feb 2023
SRR3126781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552680
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241990.9479840794772553No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45780.1793409279659025No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43710.1712318034379554No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC26710.104635128570757TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26010.10189291254681354No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25570.10016923390319195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91950.085.001111
CGTAGGG8050.077.924282
ATAGGGC16600.076.996273
TAGGGTA7500.075.4620364
ATAGCGG5650.075.1813351
TAGGGCA14350.073.9501654
ACGGGAT5400.073.365864
TAGAGGG47150.072.022442
AGGGCAT21850.071.832075
CGAAGGG20850.070.8036042
AGGGCAC18450.070.551585
GGTAAGG15550.070.416361
AGAGGGT23400.070.3425453
GTACGGG6650.069.505872
GTAGGGC13400.069.337783
AGTAGGG42700.069.145442
TAGCGGG13450.068.730712
ATAGGGA26150.068.716623
AGGGATG31750.068.6748355
AGGGTAT13400.068.033115
Produced by FastQC (version 0.11.5)