FastQCFastQC Report
Mon 27 Feb 2023
SRR3126780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555380
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232260.908905916145544No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47940.18760419194014197No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41970.16424171747450475No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC27590.10796828651707378TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG26490.10366364298069171No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25570.100063395659354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91600.085.104121
TAGGGCA15100.074.073774
ATAGCGG4400.073.776821
TAGGGTA7000.073.180084
ATAGGGC18550.072.711383
TAGGGTC6850.072.0382464
AGTAAGG14100.071.7369461
GTAGGGC14350.071.3951953
AGGGCAT23100.071.2066355
GTAGGGT8950.070.36333
CGTAGGG7550.069.751972
AGTAGGG45500.069.1353452
CGGGTAT3100.068.220555
TAACGGG9550.067.945562
TAGAGGG45000.067.2912752
ACGGGAT6500.067.2409744
TAAGGGA22450.067.1974953
CGAAGGG23250.066.940732
AGAGGGC35250.066.928183
TATAGGG22850.066.8778842
Produced by FastQC (version 0.11.5)