FastQCFastQC Report
Mon 27 Feb 2023
SRR3126779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2683850
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247050.9205059895299662No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45930.17113475045177637No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43890.1635337295303389No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC29610.11032658308027647TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG29180.10872440710173817No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG27280.10164502487098757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96450.083.093971
ACGGGTA3050.073.959034
ATAGCGG6250.073.7909551
CGTAGGG8200.073.357742
ACGGGAT7450.073.173124
TAGGGCA12800.070.125054
GAATAGG19750.069.816431
AAGGGTA17150.069.053594
ATAGGGC17250.068.925713
AGGGCAT21900.068.6682055
GTAGGGC12950.068.5870063
AATAGGG42500.068.557122
TAGAGGG51600.068.2153242
GGTAAGG15600.068.1774751
AGTAGGG46000.068.142462
CGAAGGG25150.067.8294752
ATAGGGA28350.067.79853
AGGGAAT31200.067.479715
GGGCGAT14100.067.325886
AGGGCAC18500.067.062855
Produced by FastQC (version 0.11.5)