FastQCFastQC Report
Mon 27 Feb 2023
SRR3126778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319943
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224950.96963589191631No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41270.17789230166430814No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39690.17108178950948363No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC34010.1465984293579627No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32160.13862409550579477No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG31080.13396880871642106No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG28620.12336509991840316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78900.086.623851
ACGGGTA2550.079.500114
AGTAAGG15650.076.5535661
AGGGCAT21600.075.493385
CGAAGGG19700.075.384792
AGGGATG32900.075.274495
AGTACGG2500.073.581851
GGTAAGG13000.072.5659261
GTAGGGT7350.072.482063
CGTAGGG5550.072.204692
ATAGCGG5950.072.139071
TAGCGGG13500.071.940412
AGGGAAT35100.071.225855
TAGGGCA13850.071.1436164
AGTAGGG35500.070.5189062
TAAGGGA20550.069.972513
AGGGTAG8750.069.818165
AACGAGG10150.069.706181
TAGAGGG37150.069.544242
AAGGGTA15600.069.509274
Produced by FastQC (version 0.11.5)