FastQCFastQC Report
Mon 27 Feb 2023
SRR3126777.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126777.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324759
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213990.9204825102300926No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40860.1757601540632814No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38330.16487730556156574No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC33060.14220828911728053No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG31710.13640123556893424No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31690.1363152051459958No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG29000.12474411326077241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77650.086.039751
ACGGGAT6200.082.623024
ATAGCGG4250.081.920961
AGTAAGG12350.077.335931
TAGCGGG11950.076.7335362
AGGGATG34600.074.0264055
GTACGGG4650.073.822452
CGAAGGG21050.073.272382
AGGGAAT35450.072.781735
CGTAGGG5900.072.5283662
TAGGGCA13350.072.167034
AGTAGGG35350.072.098862
AGGGCAT22300.072.075475
AGTACGG2900.071.3849951
TACGGGT1850.071.129963
TAGGGCG3900.071.09744
GTAGGGT8600.071.041343
AGAGGGC29600.070.653643
AGGGTAT13650.070.580955
TAAGGGA22050.070.548163
Produced by FastQC (version 0.11.5)