FastQCFastQC Report
Mon 27 Feb 2023
SRR3126776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448169
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225590.9214641636259587No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42270.17265964890495714No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40690.1662058460833382No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC38860.15873087192918464No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG34990.14292313970154838No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33560.13708203967945023No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG32320.13201702987007843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81500.085.353931
CGTAGGG6200.078.8299942
ATAGCGG5900.075.7841341
AGTAAGG15400.075.183241
AGAGGGC31850.074.5129553
ACGGGAT6450.074.317354
TAGCGGG13550.074.220572
CGAAGGG23250.074.181042
AGGGAAT33500.073.087445
TGTAGGG17150.072.6158752
AGGGATG37300.072.319085
TATAGGG18300.072.1614152
AGGGCAT23200.072.112725
TAACGGG7000.071.8349152
GTACGGG5100.070.9529342
AGTAGGG37050.070.7775962
TAGGGCA13300.070.668884
ATAGGGC15700.070.342543
ATAGGGA25200.070.305723
GGTAAGG14200.070.2674561
Produced by FastQC (version 0.11.5)