FastQCFastQC Report
Mon 27 Feb 2023
SRR3126775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223978
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64260.5250094364441191No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54570.44584134682159315No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41420.3384047752492283No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT29350.23979189168432763No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG27670.2260661547838278No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG26570.21707906514659578No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC24420.19951338994655135No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20170.16479054362088208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG23800.088.4554445
CGCTACG9750.088.034793
TCCGCTA9850.087.187511
CGTTTTT30400.086.922771
GCTACGA9900.085.748174
AACGAGG4750.084.4392551
GCACGGG2350.084.292082
GTAGCGG1400.084.261861
CGAAGGG10300.082.879422
AGGGCGC2800.082.2359165
CTACGAC10300.082.121865
CGTAGGG3950.081.192742
CGACCAA10250.081.147088
ACGGGAT4650.081.1380544
GTACGGG3750.080.492252
TAGCGGG4550.079.8150252
GCGTAGG2900.079.729161
AGGGCAT9550.079.71415
AGGGATT12500.079.698355
AGGGTAG3250.079.524845
Produced by FastQC (version 0.11.5)