FastQCFastQC Report
Mon 27 Feb 2023
SRR3126774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221765
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62050.5078718084083272No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC53670.43928251341297225No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41560.34016361575262016No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT28290.23155025720985623No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG28090.22991328119564727No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC26400.21608083387558166No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG26390.2159989850748712No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG19190.15706784856334893No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAA18380.15043809570580266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTA156.9044094E-493.9928368
CGTAGGG4200.090.687912
CGTTTTT30850.087.574891
TAGCGGG4950.084.547062
AGGGTAC3850.084.227355
CGCTACG8650.084.2132343
AGGGATG22850.083.709165
CGGGACG452.7284841E-1183.5491945
GCTACGA8650.083.126614
TAACGGG2800.082.290882
TCCGCTA9000.081.061031
AGGGCAT10850.080.9984365
AATGCGG2500.080.956431
GGATGGC18000.080.938287
ATTGCGG1050.080.687471
AGTAAGG6700.079.382821
CGGGTAT1900.079.151875
TACGGGA4250.078.5116653
GCGTAGG3000.078.4461441
CGACCAA8750.078.416888