FastQCFastQC Report
Mon 27 Feb 2023
SRR3126772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289696
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228480.9978617248752674No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT52400.22885134096404064No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33390.14582721898452894No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27110.11839999720486911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76750.084.624931
ACGGGAT5650.076.754914
CGTAGGG6000.075.420042
ACGGGTA2200.074.9915164
AGGGATG33150.074.849245
ATAGCGG4300.074.590451
AGGGCGA13250.071.997465
GAGGGAT28800.071.8524
CGAAGGG22950.071.271122
TAGGGCA12650.071.172084
AGGGCAT19750.070.9065255
TAGACGG3400.070.7512361
ATAGGGC14000.070.369593
AGTAAGG14750.070.031731
GGTAAGG14200.069.754741
AGTAGGG37450.069.479082
TAGAGGG36150.069.369752
TGTAGGG18600.069.185722
AGAGGGC30250.068.7195053
CGAGGGA12800.068.496723
Produced by FastQC (version 0.11.5)