FastQCFastQC Report
Mon 27 Feb 2023
SRR3126771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295173
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217290.9467260202172124No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT52090.22695456943768508No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32710.14251649004236283No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26840.11694107590146799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79250.085.5638661
TAGGGCA11650.074.226524
AAGGGTA15150.072.899144
ACGGGAT6350.072.5303044
AGGGTAC10000.072.3748865
AGGGATG30800.072.020885
GGTAAGG13750.071.5268861
AGGGCGA14550.071.383275
AAGGGCG12250.071.3582154
AGTAGGG37400.071.034792
ATAGCGG5000.070.585741
AGAGGGC30100.070.573083
ATAGGGC13950.070.41083
CGAAGGG21650.070.151822
TAGGGCG5650.069.871174
AGGGCAT19100.069.633825
CGTAGGG5350.069.4332662
TAAGGGA18500.069.351863
AGTAAGG13100.069.328491
ATAAGGG34100.069.221922
Produced by FastQC (version 0.11.5)