FastQCFastQC Report
Mon 27 Feb 2023
SRR3126770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407145
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228130.9477202245814024No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT52480.21801761007334414No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33170.13779809691564074No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28020.11640345720760487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78650.085.300061
CGTAGGG5550.078.753022
TAGGGCA12450.075.1209264
TAGGGCG6100.073.96374
ACGGGAT5350.073.790884
AGGGTAC10000.072.846545
TAAGGGA19750.071.8649443
AGGGCGA14800.071.766855
AGGGATG31950.071.489565
AGTAGGG40400.071.078312
AAGGGTA17300.070.6324844
AGGGCAT18900.070.620985
AGAGGGC32600.070.208323
AAGAGGG82050.069.709052
GAGGGAT29650.069.585224
AGGGAAT31650.069.494325
TGTAGGG19250.069.336972
ATAGCGG5850.069.162571
GTAGGGA19100.069.143313
AAGGGAT39500.068.4144744
Produced by FastQC (version 0.11.5)