FastQCFastQC Report
Mon 27 Feb 2023
SRR3126769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033576
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199920.9830957879125246No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43070.21179439568523628No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38280.18823982973835254No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26330.1294763510190915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77900.086.644351
ACGGGAT7700.082.68584
AGGGATG42400.073.702445
TAACGGG6800.072.131182
CGAGGGA11700.071.7500153
CGTAGGG6250.071.687292
GGGCGAT7750.071.549466
GTACGGG4200.070.744042
GAGGGAT33600.070.7422944
GCATAGG5850.070.1948241
TACGGGT1750.070.068563
TAAGGGA20100.069.9210743
CGGGATG9750.068.921945
AGGGATT29450.068.613445
AAGGGAT47850.068.500024
CTACGAC8750.068.205875
GGTAAGG12250.067.813831
GACCGAT1250.067.6688168
AGGGAAT29550.067.586125
ATAAGGG32250.067.417062
Produced by FastQC (version 0.11.5)