FastQCFastQC Report
Mon 27 Feb 2023
SRR3126768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032636
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188650.9281051796780142No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44440.2186323571952873No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37580.18488307793426859No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25570.1257972406274414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74350.086.0027161
ACGGGAT7300.085.624894
CGTAGGG6550.076.093852
AGGGATG44050.075.4301155
ATAGCGG5000.075.2824251
AGTACGG3150.074.6849441
TAGGGCG3750.073.942144
CGAAGGG20350.073.4763642
AAGGGAT48650.072.741694
TAAGGGA20200.072.5896153
AGTAGGG38650.071.0474242
GGGCGAT8550.070.9079366
TAGCGGG12150.070.8206252
GTAGGGC9600.070.006133
CGAGGGA13700.069.638083
GTACGGG4950.069.3432
GAGGGAT34100.069.186384
AGGGATT30100.068.543975
TAACGGG7350.068.451252
GAATAGG12850.067.739541
Produced by FastQC (version 0.11.5)