FastQCFastQC Report
Mon 27 Feb 2023
SRR3126766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861920
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204591.0988119790323967No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29800.160049841024319No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27560.14801924894732318No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21260.11418320873077255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64100.086.629081
ACGGGAT5100.078.590794
ATAGCGG3650.076.2613451
GTAGGGT6750.074.748573
TAGGGCG2400.074.661254
ATAGGGC12600.074.0998843
CGTAGGG4900.073.139532
AGGGATG28150.073.117815
AGGGCAT17050.073.0380255
AGTAAGG13400.072.880421
AGGGTAC6750.072.403095
TAGGGCA12250.072.3676764
AGTAGGG31050.071.530942
CGAAGGG21400.071.174322
GTAGGGC8700.071.0027163
TAGAGGG34000.069.069292
AAGGGTA10750.068.867464
TAGGGTC4800.068.766944
GAGGGTA10100.068.630774
AAGGGCA26950.068.5883264
Produced by FastQC (version 0.11.5)