FastQCFastQC Report
Mon 27 Feb 2023
SRR3126765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865886
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194811.0440616414936388No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31430.16844544629200284No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27400.14684712785239828No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21010.11260066263426598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69150.084.687871
AGGGCAT16600.074.4593355
TATAGGG15150.074.17452
AGGGTAC7100.074.1363455
CGTAGGG5100.073.754612
ACGGGAT4750.073.2166064
CGAAGGG16750.072.984052
ACGGGTA2150.072.1351554
AGTAAGG13050.071.006771
TAGGGCA11800.070.8944
GTAGGGC7200.070.495723
ATAGGGC12650.070.217083
AGGGATG25000.069.9317555
CGGGTAT1550.069.73775
GTAGGGT8650.069.5449143
TAAGGGA17250.069.4740453
AGTAGGG32250.069.106362
TAGAGGG35700.069.0132452
GGTAAGG11250.068.988311
TGTAGGG14150.068.1187742
Produced by FastQC (version 0.11.5)