FastQCFastQC Report
Mon 27 Feb 2023
SRR3126764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940404
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201411.0379797196872405No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32550.16774857194687293No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28550.14713430811315584No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21070.10858563474410485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68400.085.171181
AGGGCAT17000.074.087155
AGTAAGG13500.073.549011
AGGGATG26200.073.183965
CGAAGGG18750.072.68652
AACGAGG8100.072.038521
ACGGGAT5250.071.612314
TAGGGTC4550.071.268034
TAGGGCA10350.070.379854
TAAGGGA18300.070.364973
CGTAGGG4650.069.7353742
AAGGGCA27700.069.7299044
AGGGTAC7700.069.577875
GGGCGAT7500.069.553466
ATAGGGA20150.069.502153
TAGCGGG10750.069.072572
AGTAGGG31900.068.799172
ATGAGGG29600.068.588152
GGTAAGG11000.068.447161
ATGTAGG7300.068.3299561
Produced by FastQC (version 0.11.5)