FastQCFastQC Report
Mon 27 Feb 2023
SRR3126763.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126763.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011814
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213701.06222543435924No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38810.19291047780759057No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36010.17899269017911199No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23480.11671059054167035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73850.087.143691
AGGGATG30000.074.2485355
GGTAAGG11900.074.142421
TAAGGGA15050.073.3129353
ATAGGGC14650.072.417943
ATAGCGG4050.072.228521
CGAAGGG19900.071.559272
TAGGGCA10600.071.173014
GGGCGAT8700.070.759196
GAGGGAT26000.070.184124
CGTAGGG5100.069.343082
GTAAGGG31350.068.737022
AGTAGGG31450.068.518462
TAGAGGG38750.068.387492
TAGCGGG10900.067.9180452
AGATAGG13350.067.503171
ATAGAGG16150.067.4856641
TATAGGG17100.067.007322
AAGAGGG71550.066.7593152
ATAGGGA19150.066.727023
Produced by FastQC (version 0.11.5)