FastQCFastQC Report
Mon 27 Feb 2023
SRR3126762.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126762.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010778
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204611.0175663350205741No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38460.19126925001168701No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35300.17555393981831907No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23510.11691991855888617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74250.086.6389161
TAGGGCA12550.075.644244
ATAGGGC14350.075.3258743
GGTAAGG12200.074.44551
AGGGATG27250.073.987615
TAAGGGA16050.071.446843
AGTAGGG31450.070.567642
AGGGCAT19600.070.4951865
ATAGCGG4150.069.1707841
GTAGGGT7150.069.0162663
ATAGAGG16150.068.767061
GTAAGGG33150.068.225362
AAGGGTA12700.068.089844
GAGGGAT24650.067.6830144
TGTAGGG15450.067.562972
ACGGGTA2300.067.4301764
GAATAGG14450.067.412971
CGTAGGG5450.067.294942
GTAGGGC10300.067.073093
TAGCGGG10950.066.987652
Produced by FastQC (version 0.11.5)