FastQCFastQC Report
Mon 27 Feb 2023
SRR3126761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120705
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213221.0054203672835211No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39960.18842790487125743No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37330.1760263685896907No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24490.11548046522265001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74200.086.82291
AGGGATG29150.073.194465
TAAGGGA17050.072.2167363
ACGGGAT5750.071.9243244
AGTAAGG13300.070.7641141
AGTAGGG35200.070.494012
CGAAGGG21150.070.438452
ATAGGGC13500.070.319953
TAGGGCA10950.069.957524
ATAGCGG4800.069.606831
ATAGAGG14000.068.9065551
CGTAGGG5550.068.588762
GGTAAGG11600.068.558841
AGGGTAC7200.068.5358355
GTAGGGC9700.068.3137743
TAGCGGG10600.067.3904952
GGGCGAT10400.067.330816
AGGGCAT18950.066.965
GAATAGG14200.066.610461
AGAAGGG75000.066.6092
Produced by FastQC (version 0.11.5)