FastQCFastQC Report
Mon 27 Feb 2023
SRR3126760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405943
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187610.779777409523002No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36900.15337021700015338No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36580.15204017717792984No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33140.13774224908902663No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26830.11151552634455597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72300.085.62791
AGTACGG3350.077.4707261
AGGGATG41600.076.362895
GTACGGG6500.076.17862
ACGGGTA3000.075.4515154
AGGGCAT23850.073.0995255
AGTAAGG16400.073.081871
TAAGGGA22500.072.098113
ATAGGGC15250.071.740783
TAGCGGG13950.071.666912
AAGGGTA17500.071.409474
GTAGGGT7950.071.180673
AAGGGCA37550.070.955574
CGTAGGG7200.070.7372742
ATAGCGG6000.069.993641
AGGGTAT12700.069.933795
TAGAGGG43550.069.843922
AGTAGGG37700.069.548932
TAGGGCA16050.069.340174
GAGGGAT33600.069.3323064
Produced by FastQC (version 0.11.5)