FastQCFastQC Report
Mon 27 Feb 2023
SRR3126759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407424
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181100.7522563536792854No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35260.14646360591237773No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34650.14392977722245853No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31240.12976525946405787No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27190.11294229849000426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74100.085.208981
AGGGATG41250.074.282645
GTAGGGC12050.072.1523
CGAAGGG24250.071.9353562
CGTAGGG7000.071.2013242
AGTAGGG40450.070.2099462
AGTAAGG16200.069.992751
AGGGCAT21500.069.947985
ATAGGGC15250.069.646983
GAATAGG17450.069.562521
TAGGGCA13600.069.457774
TAAGGGA20950.069.0924153
AAGGGCA37400.068.986554
GAGGGAT33600.068.536284
AGAGGGC32600.068.4762043
CGAGGGA15450.068.441223
GGGCGAT10850.068.437016
GTACGGG5500.068.392352
AAGGGTA16500.068.358254
GTAAGGG37850.068.200362
Produced by FastQC (version 0.11.5)