FastQCFastQC Report
Mon 27 Feb 2023
SRR3126758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514792
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188420.7492468562012287No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36260.14418687509742356No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35950.14295416877419684No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32520.1293148697784946No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26340.10474027275416814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75350.083.6859361
AGGGATG44600.075.866435
ACGGGAT6650.073.4964
AGTAAGG15950.072.873091
ATAGGGC17500.071.969553
GGGCGAT13350.071.10866
ATAGGGA24350.071.023313
TAGGGCA15000.070.805864
AAGGGTA18150.070.686754
AGGGCAT23150.070.644815
GTAGGGC12850.070.583993
TAGGGCG4800.070.492564
CGTAGGG6850.069.978012
AGTAGGG42900.069.890062
GAATAGG16200.069.715151
AGAGGGC33200.069.21863
ACGGGTA3150.068.627684
CGAGGGA13500.068.577943
AAGGGCA37350.068.3221054
ATAGCGG5300.067.4790041
Produced by FastQC (version 0.11.5)