FastQCFastQC Report
Mon 27 Feb 2023
SRR3126757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247399
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116040.5163302110573156No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA66470.2957641255513596No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT43100.19177725005662102No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG37670.16761598630238778No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32420.1442556484184606No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28520.12690225456182902No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG27760.12352056755387006RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC27340.12165174052315589TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG26480.11782509469836021RNA PCR Primer, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50050.082.042021
ATAGCGG4250.078.8479841
CGAAGGG21550.076.6021962
TAGGGCG3850.075.954134
AGGGATG34350.074.968195
CGTAGGG6450.073.855372
GGGCGAT10700.073.7817156
AGGGCGA11900.072.659785
AGGGCAT19200.071.9563755
AGAGGGC27400.071.78043
AGTAAGG15250.071.4929661
TAGAGGG39700.070.9258042
TAGGGCA13800.070.405824
AGTACGG2350.070.29451
TAGCGGG11450.070.026732
AAGGGAC19950.069.979254
AGGGCGT3500.069.8165745
GGGTACG2900.069.677686
ATAGAGG15650.069.665671
ACGGGTA2850.069.506414
Produced by FastQC (version 0.11.5)