FastQCFastQC Report
Mon 27 Feb 2023
SRR3126756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251619
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109370.4857393724249085No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA65110.28916970411068654No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT42930.1906628075176129No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG38570.1712989631016615No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30550.13568014837323722No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28870.128218850524889No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG28740.12764148819138585RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC27590.12253405216424271TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG27570.12244522718985762RNA PCR Primer, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2600.084.956344
CGTTTTT50650.082.877331
AGGGATG34300.077.963165
TAGGGCG5050.077.24284
CGAAGGG19750.076.665722
CGTAGGG6750.076.630452
TAGCGGG10550.075.7720262
GGGCGAT10950.074.680366
AGGGCAT20600.074.374095
ATAGCGG3750.072.7858661
AAGGGAC20500.071.7565844
AGGGCGA11550.071.614665
TACGGGT1650.071.207763
ACGGGAT6250.070.683684
AGGGTAT13200.070.495695
GGTACGA605.456968E-1270.495687
AGTAGGG37200.070.2820362
TAAGGGA20150.070.204143
TAGAGGG39050.069.962862
GGTAAGG12500.069.272071
Produced by FastQC (version 0.11.5)