FastQCFastQC Report
Mon 27 Feb 2023
SRR3126755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330190
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116240.49884344195108554No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA69000.2961131924864496No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT44230.18981284787935745No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG37750.1620039567588909No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31000.13303636184173823No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG30310.13007522991687373RNA PCR Primer, Index 35 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30090.12913110089735172No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC29300.12574081941815904TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG28250.12123474909771306RNA PCR Primer, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52800.082.256761
CGTAGGG6750.076.586442
TAGGGCG4850.076.552194
TAGCGGG11800.074.875342
AGGGATG34600.074.842415
GGGCGAT10300.073.461696
ATAGCGG4750.073.3064041
AGTAGGG40100.072.07652
CGAAGGG19700.071.806412
AGGGCAT21300.070.606175
GCATAGG6750.070.4079061
AAGGGCA33150.070.1768654
GAAGGGC28850.070.0463643
AGGGCGA11750.069.995885
AAGGGAC20900.069.933684
GGGGGGA405400.069.243836
TAGTAGG11600.068.95961
AGGGTAC8750.068.750245
AGGGCGT3500.068.481685
CGAGGGA13200.068.358153
Produced by FastQC (version 0.11.5)