FastQCFastQC Report
Mon 27 Feb 2023
SRR3126754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253499
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61600.4914244048060668No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41960.3347429874295871No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25810.20590363454617835No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23300.18587968558411294No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16770.1337855076071062No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG16220.129397789707052Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG14370.11463910222505164RNA PCR Primer, Index 16 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14310.11416044209050027No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC13000.10370969581946217RNA PCR Primer, Index 16 (96% over 25bp)
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA12570.10027929818851072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG3200.083.990644
CGTAGGG3000.083.302992
CGTTTTT29600.081.454181
CGAAGGG11000.078.444852
TAGCGGG6300.077.839272
ACGGGAT3600.077.277934
GTACGGG2750.077.158872
AGGGCAT10500.076.9786155
AGGGATG20800.076.815095
CGAGGGA6950.076.665443
CGCTACG3700.076.463733
AGGGCGA7150.076.2400055
ATAGCGG2300.075.9028551
GGGCGAT5700.075.847966
TAACGGG3300.075.7299962
GTAGGGC6550.075.58823
ATTGCGG1450.074.841771
AGTAAGG8150.074.103131
CGGGTAT1400.073.8457645
TAGGGCA7550.072.4464344
Produced by FastQC (version 0.11.5)