FastQCFastQC Report
Mon 27 Feb 2023
SRR3126753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59730.4744927384260473No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42930.34103420828110176No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25870.20551024850296074No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23850.18946344904505655No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17330.1376688290126134No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG16630.1321080569232407Illumina PCR Primer Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG14150.11240703580660588RNA PCR Primer, Index 16 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13910.1105004853759638No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC13610.1081172973376612RNA PCR Primer, Index 16 (96% over 25bp)
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA13120.1042247568751003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT27700.082.55961
ATAGCGG3100.081.967781
ACGGGTA1550.081.866864
TAGGGCG3400.080.172444
CGTAGGG3550.076.8153842
AGGGATG21450.076.686125
GTACGGG3250.076.672762
ACGGGAT3900.075.919264
AGGGCAT11900.075.433185
AAGGGAC9500.075.196224
CGAAGGG10850.074.96612
TAGCGGG7850.074.267812
AGGGCGA7550.074.0757455
AGAGGGC15150.073.210843
AGGGTAC4700.072.996335
ATTGCGG1300.072.3931961
GAGGGTA6100.071.652134
GGTAAGG7300.071.550261
GTAGGGC5800.071.3067553
TAGAGGG22600.071.148582