Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126753.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1258818 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 5973 | 0.4744927384260473 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA | 4293 | 0.34103420828110176 | No Hit |
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT | 2587 | 0.20551024850296074 | No Hit |
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG | 2385 | 0.18946344904505655 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 1733 | 0.1376688290126134 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG | 1663 | 0.1321080569232407 | Illumina PCR Primer Index 7 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG | 1415 | 0.11240703580660588 | RNA PCR Primer, Index 16 (95% over 24bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 1391 | 0.1105004853759638 | No Hit |
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC | 1361 | 0.1081172973376612 | RNA PCR Primer, Index 16 (96% over 25bp) |
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA | 1312 | 0.1042247568751003 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 2770 | 0.0 | 82.5596 | 1 |
ATAGCGG | 310 | 0.0 | 81.96778 | 1 |
ACGGGTA | 155 | 0.0 | 81.86686 | 4 |
TAGGGCG | 340 | 0.0 | 80.17244 | 4 |
CGTAGGG | 355 | 0.0 | 76.815384 | 2 |
AGGGATG | 2145 | 0.0 | 76.68612 | 5 |
GTACGGG | 325 | 0.0 | 76.67276 | 2 |
ACGGGAT | 390 | 0.0 | 75.91926 | 4 |
AGGGCAT | 1190 | 0.0 | 75.43318 | 5 |
AAGGGAC | 950 | 0.0 | 75.19622 | 4 |
CGAAGGG | 1085 | 0.0 | 74.9661 | 2 |
TAGCGGG | 785 | 0.0 | 74.26781 | 2 |
AGGGCGA | 755 | 0.0 | 74.075745 | 5 |
AGAGGGC | 1515 | 0.0 | 73.21084 | 3 |
AGGGTAC | 470 | 0.0 | 72.99633 | 5 |
ATTGCGG | 130 | 0.0 | 72.393196 | 1 |
GAGGGTA | 610 | 0.0 | 71.65213 | 4 |
GGTAAGG | 730 | 0.0 | 71.55026 | 1 |
GTAGGGC | 580 | 0.0 | 71.306755 | 3 |
TAGAGGG | 2260 | 0.0 | 71.14858 | 2 |