FastQCFastQC Report
Mon 27 Feb 2023
SRR3126752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318036
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60710.4606095736383528No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44190.3352715707309967No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26070.19779429393430834No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24220.18375825849976782No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG17950.13618747894594685Illumina PCR Primer Index 7 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17770.13482181063339696No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG15870.12040642288981485RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC14460.10970868777484075RNA PCR Primer, Index 16 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14230.10796366715324923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT26850.082.8830951
CGTAGGG4100.082.525232
CGAAGGG12500.080.452932
ATAGCGG3250.078.173491
TAGGGCG2000.077.539334
AGGGATG20950.076.490675
AGGGCAT12650.075.7840355
TAGCGGG7450.075.063492
CAGGGTA3500.073.846984
GCGTAGG2550.073.802131
GGGCGAT5800.073.731236
CGAGGGA6450.072.858193
AAGGGTA8550.072.5514144
ATAGAGG8900.072.423531
AGTAAGG9700.072.2709351
ACTAGGG5950.071.872462
AGAGGGC14000.071.832973
AAGGGAC12500.071.8061144
AGAAGGG41100.071.805212
AGGGTAC4800.071.469335