Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126752.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1318036 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 6071 | 0.4606095736383528 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA | 4419 | 0.3352715707309967 | No Hit |
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT | 2607 | 0.19779429393430834 | No Hit |
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG | 2422 | 0.18375825849976782 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG | 1795 | 0.13618747894594685 | Illumina PCR Primer Index 7 (95% over 21bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 1777 | 0.13482181063339696 | No Hit |
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG | 1587 | 0.12040642288981485 | RNA PCR Primer, Index 16 (95% over 24bp) |
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC | 1446 | 0.10970868777484075 | RNA PCR Primer, Index 16 (96% over 25bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 1423 | 0.10796366715324923 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 2685 | 0.0 | 82.883095 | 1 |
CGTAGGG | 410 | 0.0 | 82.52523 | 2 |
CGAAGGG | 1250 | 0.0 | 80.45293 | 2 |
ATAGCGG | 325 | 0.0 | 78.17349 | 1 |
TAGGGCG | 200 | 0.0 | 77.53933 | 4 |
AGGGATG | 2095 | 0.0 | 76.49067 | 5 |
AGGGCAT | 1265 | 0.0 | 75.784035 | 5 |
TAGCGGG | 745 | 0.0 | 75.06349 | 2 |
CAGGGTA | 350 | 0.0 | 73.84698 | 4 |
GCGTAGG | 255 | 0.0 | 73.80213 | 1 |
GGGCGAT | 580 | 0.0 | 73.73123 | 6 |
CGAGGGA | 645 | 0.0 | 72.85819 | 3 |
AAGGGTA | 855 | 0.0 | 72.551414 | 4 |
ATAGAGG | 890 | 0.0 | 72.42353 | 1 |
AGTAAGG | 970 | 0.0 | 72.270935 | 1 |
ACTAGGG | 595 | 0.0 | 71.87246 | 2 |
AGAGGGC | 1400 | 0.0 | 71.83297 | 3 |
AAGGGAC | 1250 | 0.0 | 71.806114 | 4 |
AGAAGGG | 4110 | 0.0 | 71.80521 | 2 |
AGGGTAC | 480 | 0.0 | 71.46933 | 5 |