FastQCFastQC Report
Mon 27 Feb 2023
SRR3126751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985022
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122130.6152576646505681No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39950.20125721528527138No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38480.19385175579918007No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23680.11929338818411081No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21570.10866378307142188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46350.082.9941561
ACGGGAT5800.078.0782854
CGTAGGG6300.077.1249162
AGGGATG31900.074.685855
GAATAGG13950.073.421831
ATAGGGC13400.072.518613
GGTAAGG13750.072.086881
AGTAGGG33100.071.68662
ATAGCGG3850.071.106111
AGGGTAC8900.070.7515265
AGGGTAT10500.070.71135
AGGGCAC14500.070.325495
TAAGGGA18000.069.972243
AAGGGCA28300.069.841674
AAGGGTA13050.069.76444
AATAGGG33500.069.422482
AGAGGGC26850.069.396863
TGTAGGG16450.069.111312
TAGAGGG37100.068.916532
AGAAGGG72000.068.794672
Produced by FastQC (version 0.11.5)