FastQCFastQC Report
Mon 27 Feb 2023
SRR3126750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996552
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119750.5997840276636922No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39800.19934366848446722No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39190.1962884012036751No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22170.11104143543468939No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21850.10943867227099519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47550.081.333951
ATAGGGC12850.076.438983
AGGGATG31850.074.369155
AGTAGGG34200.074.250692
CGTAGGG6300.072.4042362
TAGCGGG9500.071.280662
GAATAGG14400.070.246791
GGGTACG1550.069.737736
AGTAAGG13950.069.140131
ACGGGAT5650.069.040094
TGTAGGG15850.068.832182
AGGGACT30450.068.682245
AGGGCAT18300.068.569645
AGCGGGG31500.068.48163
AAGGGCA28700.068.285084
TAAGGGA19150.068.2256553
CGAGGGA11300.068.208283
ACGGGTA2000.068.14594
GAGGGAT27700.067.865954
AGGGCAC14900.067.8147055
Produced by FastQC (version 0.11.5)