FastQCFastQC Report
Mon 27 Feb 2023
SRR3126749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097644
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126310.6021517473889755No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41360.19717359094298176No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39530.1884495176493247No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24850.11846624117343077No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22430.10692948851187332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49450.081.864971
CGTAGGG6250.078.953372
ATAGCGG5350.076.547571
AGGGATG30700.076.234645
ATAGGGC14450.074.803413
ACGGGAT5100.074.640794
ACGGGTA2150.074.319344
AGGGTAC9650.074.024885
AAGGGTA14200.073.141754
AGGGCAC15000.072.3739245
TAGGGCG5350.071.152914
GAATAGG13000.070.970631
AGGGCAT18150.070.947215
ATGAGGG34700.070.832672
TAGGGCA13000.070.494094
TAAGGGA18650.070.305093
GTAGGGT9050.070.104613
GAGGGAT26050.069.9979554
TAGCGGG11800.069.697542
AGTAGGG35600.069.437992
Produced by FastQC (version 0.11.5)