FastQCFastQC Report
Mon 27 Feb 2023
SRR3126747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303343
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139510.6056848676033053No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT76550.33234303358205874No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA50000.21707578940696198No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT34500.14978229469080376No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29020.12599078817180073No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG28350.12308197259374744No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC24230.10519492754661378No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG23440.10176513007398376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50050.081.701171
TAGGGCG5050.075.3805164
CGAAGGG22150.073.453592
AGGGATG29550.072.999635
CGTAGGG6250.072.979752
GGTAAGG12500.072.654061
AGTAGGG36300.072.283342
GGGCGAT10600.072.268196
AGTAAGG12650.071.792561
AGGGCAT19650.071.511215
GAATAGG14800.070.901561
TGTAGGG18200.070.2761842
ATAGGGC15800.070.1972963
AGAGGGC30300.070.106983
TAGGGCA12900.069.9482654
GTAAGGG33150.069.789842
AGACGGG14550.069.161032
ATAGCGG4900.069.1431351
TAAGGGA16550.068.719953
TAACGGG7900.068.451252