FastQCFastQC Report
Mon 27 Feb 2023
SRR3126746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439505
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147950.6064754940039065No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT79680.3266236388119721No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA53410.218937858295023No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT35410.14515239772002925No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG30590.12539429105494762No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28360.11625309232815674No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC25760.10559519246732432No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG25290.10366857210786615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53350.082.9112241
CGTAGGG7200.075.713852
ATAGCGG4900.074.906321
AGTAAGG14100.073.755271
AGGGCAT21800.072.001215
AGAGGGC31500.071.760323
AGTAGGG41250.070.9763952
AGGGATG29450.069.89385
ACGGGAT6150.069.537034
ATGAGGG34950.069.382892
TAGAGGG44100.069.160162
TAAGGGA19550.068.749433
GAATAGG14800.068.67711
AGGGAAT28300.068.2504045
AGGGTAT12550.068.151835
GGGCGAT11600.068.061446
AAGGGCA35100.068.0152744
AGGGCAC15000.067.985825
AAGAGGG79400.067.7695852
GGTAAGG12950.067.223611
Produced by FastQC (version 0.11.5)