FastQCFastQC Report
Mon 27 Feb 2023
SRR3126745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528245
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165420.6542878558051138No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT58990.23332390650431428No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41060.16240514665311312No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG30290.1198064269878908Illumina PCR Primer Index 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC28400.11233088565388244TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG27980.11066965424632502RNA PCR Primer, Index 10 (95% over 22bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26060.10307545352606254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2350.082.301394
ACGGGAT5950.080.867574
CGTTTTT65050.080.779541
CGTAGGG6500.080.558142
AGGGCAT21400.074.87945
GTAGGGC12200.073.852423
TAGGGCG5150.073.278054
AGTAAGG14450.071.880041
GGGCGAT13150.071.827716
TAAGGGA19150.071.436553
GAATAGG16900.071.237221
CGAAGGG27550.071.2314152
TATAGGG20600.070.760532
AAGGGAC24700.070.6636354
ATAGCGG4950.070.5797351
AGAGGGC34600.069.804763
AGTACGG3250.069.728671
AGTAGGG40700.069.311712
TAGCGGG12650.068.616272
AAGGGCA36800.068.323584
Produced by FastQC (version 0.11.5)