Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126744.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2533763 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 15704 | 0.6197896172609672 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 5802 | 0.22898747830795538 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA | 3944 | 0.155657810142464 | No Hit |
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG | 3102 | 0.12242660422462559 | Illumina PCR Primer Index 2 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG | 2808 | 0.110823309046663 | RNA PCR Primer, Index 10 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC | 2763 | 0.1090472944786075 | TruSeq Adapter, Index 10 (95% over 23bp) |
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG | 2610 | 0.10300884494721882 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 6210 | 0.0 | 81.929825 | 1 |
CGTAGGG | 745 | 0.0 | 76.38098 | 2 |
GTAGGGC | 1255 | 0.0 | 74.14573 | 3 |
AGAGGGC | 3520 | 0.0 | 73.16486 | 3 |
ATAGCGG | 530 | 0.0 | 72.8192 | 1 |
CGAAGGG | 2390 | 0.0 | 72.41125 | 2 |
AGGGCAT | 2475 | 0.0 | 71.58645 | 5 |
AGTACGG | 250 | 0.0 | 71.54042 | 1 |
AGTAAGG | 1670 | 0.0 | 70.45818 | 1 |
TAGGGCA | 1435 | 0.0 | 70.085236 | 4 |
TACGGGT | 175 | 0.0 | 69.823235 | 3 |
TAAGGGA | 1915 | 0.0 | 69.69702 | 3 |
TATAGGG | 2125 | 0.0 | 69.49072 | 2 |
ATAGGGC | 1475 | 0.0 | 69.45909 | 3 |
ACGGGAT | 575 | 0.0 | 68.655624 | 4 |
GTAAGGG | 3890 | 0.0 | 68.54718 | 2 |
AGTAGGG | 4045 | 0.0 | 68.245766 | 2 |
AGGGATG | 2845 | 0.0 | 67.892876 | 5 |
AGGGACT | 4315 | 0.0 | 67.5267 | 5 |
GTACGGG | 440 | 0.0 | 67.335495 | 2 |