FastQCFastQC Report
Mon 27 Feb 2023
SRR3126744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533763
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157040.6197896172609672No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT58020.22898747830795538No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39440.155657810142464No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG31020.12242660422462559Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG28080.110823309046663RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC27630.1090472944786075TruSeq Adapter, Index 10 (95% over 23bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26100.10300884494721882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62100.081.9298251
CGTAGGG7450.076.380982
GTAGGGC12550.074.145733
AGAGGGC35200.073.164863
ATAGCGG5300.072.81921
CGAAGGG23900.072.411252
AGGGCAT24750.071.586455
AGTACGG2500.071.540421
AGTAAGG16700.070.458181
TAGGGCA14350.070.0852364
TACGGGT1750.069.8232353
TAAGGGA19150.069.697023
TATAGGG21250.069.490722
ATAGGGC14750.069.459093
ACGGGAT5750.068.6556244
GTAAGGG38900.068.547182
AGTAGGG40450.068.2457662
AGGGATG28450.067.8928765
AGGGACT43150.067.52675
GTACGGG4400.067.3354952