FastQCFastQC Report
Mon 27 Feb 2023
SRR3126743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687572
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167790.6243181578019119No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT60500.22511024820916428No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41070.15281451064380785No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG34030.12661986357946875Illumina PCR Primer Index 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC30780.11452716429550538TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG29400.1093924181380071RNA PCR Primer, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66000.081.636141
ACGGGTA3500.075.193974
CGTAGGG7100.074.795432
TAGGGCA14750.072.32644
TACGGGT2600.072.300553
ACGGGAT5850.070.695184
AGAGGGC37650.070.524243
AGTAAGG17950.070.5194241
TAGGGCG4600.070.494354
AGTAGGG41700.070.436692
GTAGGGC11800.070.0947653
AGGGCAT23700.069.9999165
GGGCGAT12850.069.855636
AAGGGTA17100.069.8072744
GTAAGGG40650.069.481452
CGAAGGG27000.068.7524642
ACGGGTC3100.068.220344
TAAGGGA21800.068.121713
ATAGGGC16150.067.219363
AGGGACT43400.067.1387255
Produced by FastQC (version 0.11.5)