FastQCFastQC Report
Mon 27 Feb 2023
SRR3126742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309633
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180820.7828949447812705No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41760.18080794654388815No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37450.1621469731338269No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36040.15604210712264677No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT29760.12885164006575936No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24770.10724647595527083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67400.084.672821
CGTAGGG6600.078.562012
AGGGATG29300.076.664885
ACGGGTA3150.076.315734
ACGGGAT5250.075.417894
AGGGCAT21050.075.010725
TAGGGCA12050.073.14914
GTAGGGT9100.072.5172043
AGAGGGC29300.072.393463
ATAGCGG4700.072.252551
ATAGGGC14300.071.8579563
GGGCGAT12850.071.312836
GTAGGGC11400.071.117753
TAAGGGA21500.071.0331343
AGTAAGG13550.071.008351
TAGGGCG4650.070.9576954
GTACGGG5450.070.9220352
AGTAGGG36900.070.641942
AGGGAAT31150.070.603125
TAGAGGG37750.070.1751252
Produced by FastQC (version 0.11.5)