FastQCFastQC Report
Mon 27 Feb 2023
SRR3126741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326865
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173530.7457673736980873No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40940.17594488721949922No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37170.15974282994501185No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34910.15003019083616798No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT29370.12622133213572767No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23910.10275628366922876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64450.085.788421
ATAGCGG4050.079.007381
AGGGCAT19400.076.06935
ACGGGAT5550.075.366534
ATAGGGC15100.075.3216553
AGGGATG28250.075.03085
CGAAGGG24350.074.539162
CGTAGGG6750.074.5377352
GTAGGGT7900.072.5795443
TAGCGGG12600.072.398132
TAAGGGA21650.072.288263
AGTAGGG36700.072.261882
AGAGGGC28350.071.94793
ATGAGGG36050.070.173512
TAGGGCA13400.070.146614
CTACGAC8000.069.909865
GGGCGAT11700.069.8948066
TATAGGG18650.069.33462
TAGGGTC5700.069.2605444
AGACGGG17300.069.03732
Produced by FastQC (version 0.11.5)