FastQCFastQC Report
Mon 27 Feb 2023
SRR3126740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503177
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184710.737902273790467No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44850.1791723078312081No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39150.15640124529747598No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35300.14102079077907795No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT31250.12484135161037353No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25160.10051226900854393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70450.084.9641651
ACGGGTA3450.081.7346654
AGGGCAT23350.076.282775
ATAGCGG3950.076.245221
CGTAGGG6200.075.044292
AGGGATG31400.074.537335
TCCGCTA9050.073.3162461
GACCGAT1800.073.107128
CTACGAC9200.072.539515
GAATAGG16500.072.154971
ACGGGAT6700.071.5483254
AGAGGGC32150.071.190513
ATAGGGC14700.070.6560063
AGTAGGG39300.069.958012
AGTAAGG15450.069.4442141
GGGCGAT11450.069.3673866
GAGGGAT28300.069.250634
TACGGGT2250.068.9295653
TAGCGGG13100.068.522982
AAGGGAC19900.068.488734
Produced by FastQC (version 0.11.5)