FastQCFastQC Report
Mon 27 Feb 2023
SRR3126739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706324
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83780.4909970204955214No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA51430.3014081733598074No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44260.25938801775043896No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG36940.21648877938773645No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27910.16356799763702556No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG27360.16034469420813396No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG24460.14334909431034198No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC22120.12963540335833054TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA2650.085.118835
CGTTTTT38900.082.2278751
CGAAGGG15050.081.469862
CGCTACG9000.081.215053
TAGGGCG3950.081.181894
AGGGATG29750.081.0327765
ATAGCGG3050.080.434521
ACGGGAT5050.080.307194
AGTACGG1850.079.0549241
AGGGCAT18500.077.728455
TCCGCTA9450.077.3818051
AAGGGAC14950.077.2809144
AGGGTAC6650.077.025615
TAGCGGG7650.076.439952
AGTAAGG10700.076.278341
AGAGGGT13550.075.869033
AAGGGCA24200.075.60734
GCTACGA9700.075.354174
CGGGTAT2500.075.188315
GAAGGGC19100.075.0563663
Produced by FastQC (version 0.11.5)