FastQCFastQC Report
Mon 27 Feb 2023
SRR3126738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707860
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79000.462567189348073No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49900.2921785158034031No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43620.2554073518906702No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG35360.20704273183984634No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27580.1614886466103779No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG27280.1597320623470308No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG24650.14433267363835445No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC23020.1347885658075018TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG2600.085.052161
TAGCGGG5900.082.87512
CGAAGGG14750.082.87512
CGTTTTT37050.081.9091341
AGGGCAT18200.081.861185
CTAACGG700.080.6573641
CGCTACG7950.079.8099063
AGCGGGA9150.079.616073
AGGGATG30550.078.613995
AAGGGCA24700.078.395374
ACGGGAT5700.077.507394
CGGGATA3100.077.321215
TAACGGG5050.077.27332
AGTAAGG11500.076.916731
CGTAGGG4150.075.904842
AGAGGGT14350.075.657193
CGAGGGA9550.075.29713
TACGCGG750.075.280211
ACGGGTA2500.075.198674
AGAGGGC19750.074.246783
Produced by FastQC (version 0.11.5)